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Tersedia di Perpustakaan Utama UIN Mahmud Yunus Batusangkar
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A practical guide for those working in the field of trauma - from the result of war to the aftermath of sexual abuse. It aims to help the sufferer or group, and discusses how they can discover new paths, or be reminded of long-forgotten coping skills.
A practical guide for those working in the field of trauma - from the result of war to the aftermath of sexual abuse.
Luvunga scandens belong to the family of Rutaceae which usually inhabits tropical and moist environment. This plant is known as 'Mengkurat Jakun' among locals and used traditionally to treat fever and fatigue via decoction. The study aimed to elucidate the compounds from L. scandens stem, which were isolated using bioactivity guided isolation together with their mechanism of action on human breast cancer cell (MCF-7). The bioactivity-guided isolation of cytotoxic agent of the stem of L. scandens resulted in the isolation and characterization of a new triterpenoid from this species, 3-oxotirucalla-7,24-dien-21-oic-acid (2), along with a known triterpenoid, flindissol (1). The isolation was conducted using chromatographic techniques on silica gel and sephadex LH-20. The structures of the isolated compounds were elucidated on the basis of spectroscopic analysis including UV, IR, NMR, MS and 2D NMR. The cytotoxic activity of the plant on the proliferation of MCF-7 cell line was evaluated by MTT, WST-1 assay, scanning electron microscope (SEM), flow cytometry and RT-qPCR. The cytotoxic evaluation of the extracts showed that IC50 value of the dichloromethane (LSC-SD) and methanol (LSC-SM) extracts from the stem were 75.0 and 77.0 μg/mL respectively. Whereas IC50 value of n-hexane (LSF-H), n-hexane:DCM (LSF-HD), DCM (LSF-D) and DCM:MeOH (LSF-DM) fraction extracts were 97.5, 35, 98.9 and 94 μg/mL respectively. The IC50 value of MeOH (LSF-M) fraction could not be extrapolated since none of the concentration of this extract was able to reduce the proliferation activity to 50 %. Compound 1 and 2 showed potent cytotoxicity against MCF-7 cell line with IC50 values of 13.8 μM and 27.5 respectively after 24 h of treatment. Doxorubicin was used as a positive control drug with IC50 values of 6.21 μM. Morphological analysis of the cells surface exhibit the apoptosis results after 24 h treated with LSC-SD extract, 1 and 2, where the cells were rounded up, shrank and lost contact with neighboring cells. Apoptosis of MCF-7 cells treated with both compounds were confirmed by flow cytometry. The results show that compound 1 and 2 exert their anti-proliferative effect on MCF-7 cells through inhibiting cell cycle where the distribution of cells at Sub G1 phase was 7.7 and 9.3 % respectively. Further evident of apoptosis induction by compound 1 and 2 towards MCF-7 cell lines were assayed by RT-qPCR on the expression of PUMA, caspase-8 and caspase-9. In this assay, both compounds showed the increased of the expression of PUMA, caspase-8 and caspase-9 gene. In conclusion, both triterpenoids, 3-oxotirucalla-7,24-dien-21-oic-acid (2) and flindissol (1) have a potential to be developed as anticancer agents against breast cancer.
Luvunga scandens belong to the family of Rutaceae which usually inhabits tropical and moist environment.
Explores research on readers' advisory services and examines the needs of adults, young adults, and children in a public library setting, discussing the practical aspects of each topic.
Explores research on readers' advisory services and examines the needs of adults, young adults, and children in a public library setting, discussing the practical aspects of each topic.
This special issue of Assistive Technology Outcomes and Benefits focuses on research related to assistive technology applications for children with autism.
Although many teachers and/or direct care providers often operate under the
assumption that working with video requires too much time, it can actually be
comparable to or require less time than creating picture-based visual supports as
was ...
The quality of the academics who undertake the work of teaching and research is critical to the significance, status and relevance of our universities. There is widespread evidence that doctoral students are not being properly prepared for the changing face of higher education and that once they take up academic positions, they often experience many frustrations and tensions. This book, based on a four-year-long research program conducted by four academics and four graduate students, investigates the experiences of doctoral students, new academics and senior academics as they engage in their work related to doctoral education. Doctoral Education: Research-Based Strategies for Doctoral Students, Supervisors and Administrators offers research-based strategies for improving doctoral education in a non-technical and conversational way. Those strategies include learning to be a new supervisor alongside other academic work, developing an intellectual network during the doctoral journey, giving and receiving feedback on scholarly writing, and preparing for the oral defence. Also, based on research evidence, the book challenges taken-for-granted practices and policies surrounding doctoral education, including the gendered nature of disciplinary practices, the paradox of writing in doctoral education and the public oversight of more and more aspects of academic work. Intended for doctoral students, academics, staff and administrators, this book provides several perspectives on the topic of doctoral education and contains the actual voices of doctoral students and new academics to illustrate its discussion.
Research-Based. Strategies. Policies and practices within institutions are often
built on taken-for-granted practices (without the benefit of evidence) or result from
external calls for accountability (perhaps accepted without evidence to challenge
...
Colonies which exhibited significantly larger halo on the YEPG plates compared to the parent strain were isolated. Their acid production ability were reconfirmed on similar plates. Instead of growing isolated strains as shake flask cultures and assaying for their citric acid production capability, a more rapid method was developed. This involved removing agar plugs from the halo area of isolates grown on the YEPG plates using a cork borer. These plugs were then subjected to a 'freeae squeeze' regime to obtain samples for citric acid analysis by HPLC. [Authors' abstract].
Colonies which exhibited significantly larger halo on the YEPG plates compared to the parent strain were isolated.
