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Creation of a Framework Genetic Linkage Map of Colonial Bentgrass and the Identification of Genomic Regions Associated with Dollar Spot Resistance

By comparing phenotypic data obtained from field trails we identified regions potentially associated with dollar spot resistance in colonial bentgrass. The segregation of resistance in the backcross population suggests a three gene recessive epistasis model for dollar spot resistance in colonial bentgrass. By comparing genotypes we identified colonial bentgrass loci on groups 2A1 and 3A1 that are common in all resistant individuals. We hypothesize that genes related to dollar spot resistance may lie on these groups. Other resistant backcross individuals not included in the mapping population also shared these loci proportions significantly higher then expected by random chance (p

Methods Selection of initial amplification primers Unigenes contained within the
creeping and colonial bentgrass libraries were determined using the CAP3
program as described in Chapter 2 (Huang & Madan, 1999). Unigenes which ...